The two copper ions bound in the active site of Octopus vulgaris haemocyanin can be removed by cyanide. The two metal ions react with the ligand sequentially. In this paper the preparation of Octopus half-apo-haemocyanin, containing at the active site a single copper ion, is described. Moreover, the conditions to obtain Octopus apohaemocyanin, containing less than 3% of copper- still bound, are given.
Removal of copper from Octopus vulgaris hemocyanin. Preparation of half-apo and apo-derivatives.
BELTRAMINI, MARIANO;
1984
Abstract
The two copper ions bound in the active site of Octopus vulgaris haemocyanin can be removed by cyanide. The two metal ions react with the ligand sequentially. In this paper the preparation of Octopus half-apo-haemocyanin, containing at the active site a single copper ion, is described. Moreover, the conditions to obtain Octopus apohaemocyanin, containing less than 3% of copper- still bound, are given.File in questo prodotto:
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