Micronuclei are indirect but reliable indicators of chromosome damage, formed at the mitotic division after either chromosome loss or chromosome breakage events, and visible in the cytoplasm of interphase cells as small bodies of extra-chromatin. Centromeric, pericentromeric, or telomeric repetitive sequences can be considered as indicators of chromosome integrity into the micronucleus. Therefore the localisation of these sequences may contribute to predict the mechanism of induction of micronuclei following the exposure of mammalian cells to mutagens. We review here the results obtained in our laboratory by application of different PRINS (PRimed In Situ labelling) strategies for characterising the micronucleus content; the performance of PRINS is compared with that of the other procedures (kinetochore immunodetection, Fluorescence In Situ Hybridisation) applicable to the same goal.

PRINS detection of centromeric and telomeric sequences in micronuclei: an useful tool to distinguish chromosome breakage from aneuploidy

RUSSO, ANTONELLA
2007

Abstract

Micronuclei are indirect but reliable indicators of chromosome damage, formed at the mitotic division after either chromosome loss or chromosome breakage events, and visible in the cytoplasm of interphase cells as small bodies of extra-chromatin. Centromeric, pericentromeric, or telomeric repetitive sequences can be considered as indicators of chromosome integrity into the micronucleus. Therefore the localisation of these sequences may contribute to predict the mechanism of induction of micronuclei following the exposure of mammalian cells to mutagens. We review here the results obtained in our laboratory by application of different PRINS (PRimed In Situ labelling) strategies for characterising the micronucleus content; the performance of PRINS is compared with that of the other procedures (kinetochore immunodetection, Fluorescence In Situ Hybridisation) applicable to the same goal.
PRINS and PNA technologies in chromosomal investigation
9781600214134
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/1559797
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