Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system

Colavita, A.; Capello, G.; Ijaduola, R. B.; Cunei, A.; Ying, L.; Fratnik, F.; Lagostena, L.; Canepari, M.; Mammano, Fabio

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This paper describes results obtained with a novel imaging system based on a fast CCD device. Sensor’s output was digitized at 12 bit/pixel by customized electronics, reaching acquisition rates as high as 800 frames/s with a full-frame resolution of 128´128 pixels. The software developed for the project permitted the sequential capture of thousands of images directly to host PC RAM without frame loss even at the maximum readout rate (16 MHz). It is shown that the high spatio-temporal resolution of this apparatus is of value when investigating the time-course of rapid intracellular Ca2+ fluorescence transients, particularly those associated with neuronal action potentials near physiological temperature.

Titolo:	Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system
Autori:	COLAVITA A. CAPELLO G. IJADUOLA R. B. CUNEI A. YING L. FRATNIK F. LAGOSTENA L. CANEPARI M. MAMMANO, FABIO mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	1999
Abstract:	This paper describes results obtained with a novel imaging system based on a fast CCD device. Sensor’s output was digitized at 12 bit/pixel by customized electronics, reaching acquisition rates as high as 800 frames/s with a full-frame resolution of 128´128 pixels. The software developed for the project permitted the sequential capture of thousands of images directly to host PC RAM without frame loss even at the maximum readout rate (16 MHz). It is shown that the high spatio-temporal resolution of this apparatus is of value when investigating the time-course of rapid intracellular Ca2+ fluorescence transients, particularly those associated with neuronal action potentials near physiological temperature.
Handle:	http://hdl.handle.net/11577/174034
ISBN:	0819430749
Appare nelle tipologie:	04.01 - Contributo in atti di convegno

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