We are presently exploring the links between neuronal regeneration, development and the stress response in echinoderms. During echinoderm development there is known to be an increase in HMW ubiquitin-protein conjugates together with a parallel increase in ubiquinated H2A and H2B histones. This could reflect the high levels of protein turnover experienced during ontogeny. In contrast, the stress response (or "heat-shock" response) induced either by temperature or chemical stress produces an increase in ubiquinated HMW proteins, induction of heat-shock proteins (hsp72 and hsp90) and a decrease in ubiquinated histones. We have analysed these parameters in crinoids and asteroids during various phases of arm regeneration. Normal arms of Antedon mediterranea, Antedon bifida and Asterias rubens were compared with regenerating arms at different stages in both standard and conditions of environmental stress. In normal arms we detect significant quantities of ubiquinated HMW proteins and histones, perhaps indicating rapid cellular growth and protein turnover. By western blot analysis and immunocytochemical labelling of neurones, using monoclonal antibodies against ubiquitin and the inducible form of hsp70, we found significant ubiquitin changes at early stages of regeneration. In particular a decrease in ubiquitinated histones for both crinoids and asteroids with different timing of deubiquitination was observed. No other significant changes were noticed during the rest of the regenerative period. The expression of hsp70, instead, was induced also in advanced phases of regeneration confirming the chaperon role of this molecule. In conclusion, it is clear that ubiquitin dynamics are important in regeneration and furthermore hsp 70 is an important component of regeneration rather than a response to stress alone. (Supported by a Thomas Holloway studentship, RHUL strategy fund, University of Milan, The British Council and the University of London Central Research Fund).

Stress and neuronal regeneration in echinoderms.

PATRUNO, MARCO VINCENZO;
1999

Abstract

We are presently exploring the links between neuronal regeneration, development and the stress response in echinoderms. During echinoderm development there is known to be an increase in HMW ubiquitin-protein conjugates together with a parallel increase in ubiquinated H2A and H2B histones. This could reflect the high levels of protein turnover experienced during ontogeny. In contrast, the stress response (or "heat-shock" response) induced either by temperature or chemical stress produces an increase in ubiquinated HMW proteins, induction of heat-shock proteins (hsp72 and hsp90) and a decrease in ubiquinated histones. We have analysed these parameters in crinoids and asteroids during various phases of arm regeneration. Normal arms of Antedon mediterranea, Antedon bifida and Asterias rubens were compared with regenerating arms at different stages in both standard and conditions of environmental stress. In normal arms we detect significant quantities of ubiquinated HMW proteins and histones, perhaps indicating rapid cellular growth and protein turnover. By western blot analysis and immunocytochemical labelling of neurones, using monoclonal antibodies against ubiquitin and the inducible form of hsp70, we found significant ubiquitin changes at early stages of regeneration. In particular a decrease in ubiquitinated histones for both crinoids and asteroids with different timing of deubiquitination was observed. No other significant changes were noticed during the rest of the regenerative period. The expression of hsp70, instead, was induced also in advanced phases of regeneration confirming the chaperon role of this molecule. In conclusion, it is clear that ubiquitin dynamics are important in regeneration and furthermore hsp 70 is an important component of regeneration rather than a response to stress alone. (Supported by a Thomas Holloway studentship, RHUL strategy fund, University of Milan, The British Council and the University of London Central Research Fund).
1999
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/187492
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