Longitudinal studies were conducted in commercial meat turkey farms in order to investigate on the occurrence of thermophilic Campylobacter species and to evaluate the trend of infection during the entire production cycle (i.e. from day-old chicks up to slaughter time). Furthermore, Campylobacter species distribution analysis and genetic characterization of isolates were carried out, and their antimicrobial susceptibility profile was determined. Three flocks belonging to three different intensive farms located in the province of Vicenza, Veneto region, were examined. Samples were regularly collected at weekly intervals up to Campylobacter colonization of the flocks, then twice a month up to time of slaughter. A conventional culture method and a multiplex end-point PCR assay were used for Campylobacter detection and identification, respectively. Selected isolates were genetically characterized by Random Amplified Polymorphic DNA (RAPD)–PCR and by flaA short variable region (SVR) DNA sequencing. Antimicrobial susceptibility testing of isolates was performed by disk diffusion method. A high occurrence of thermophilic Campylobacter infection was detected, with colonization of birds persisting throughout the whole production cycle. Both C. jejuni and C. coli were recovered and a high genetic diversity was observed among them. Moreover, antimicrobial-resistance appeared to be widely spread among turkey campylobacters.
Monitoraggio longitudinale per il rilievo di Campylobacter termofili in allevamenti di tacchini da carne
GIACOMELLI, MARTINA;MARTINI, MARCO;PICCIRILLO, ALESSANDRA
2011
Abstract
Longitudinal studies were conducted in commercial meat turkey farms in order to investigate on the occurrence of thermophilic Campylobacter species and to evaluate the trend of infection during the entire production cycle (i.e. from day-old chicks up to slaughter time). Furthermore, Campylobacter species distribution analysis and genetic characterization of isolates were carried out, and their antimicrobial susceptibility profile was determined. Three flocks belonging to three different intensive farms located in the province of Vicenza, Veneto region, were examined. Samples were regularly collected at weekly intervals up to Campylobacter colonization of the flocks, then twice a month up to time of slaughter. A conventional culture method and a multiplex end-point PCR assay were used for Campylobacter detection and identification, respectively. Selected isolates were genetically characterized by Random Amplified Polymorphic DNA (RAPD)–PCR and by flaA short variable region (SVR) DNA sequencing. Antimicrobial susceptibility testing of isolates was performed by disk diffusion method. A high occurrence of thermophilic Campylobacter infection was detected, with colonization of birds persisting throughout the whole production cycle. Both C. jejuni and C. coli were recovered and a high genetic diversity was observed among them. Moreover, antimicrobial-resistance appeared to be widely spread among turkey campylobacters.Pubblicazioni consigliate
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