An AMPV reverse-genetics system was used to create a virus lacking the entire SH open reading frame which produced unusual giant syncytial formations in cell culture. The replacement of the gene with GFP resulted in virus which also produced the same syncytial formations. The intrinsic fluorescent properties of the virus may prove useful in pathogenesis studies. The similarity of size of the 2 genes suggests that the altered cytopathic effect did not result from the shortening of the genome and possible consequential effects on the transcription or translation of the following attachment and polymerase protein genes.

Construction of GFP Avian Metapneumovirus (AMPV) recombinant lacking the small hydrophobic protein gene

CECCHINATO, MATTIA;
2009

Abstract

An AMPV reverse-genetics system was used to create a virus lacking the entire SH open reading frame which produced unusual giant syncytial formations in cell culture. The replacement of the gene with GFP resulted in virus which also produced the same syncytial formations. The intrinsic fluorescent properties of the virus may prove useful in pathogenesis studies. The similarity of size of the 2 genes suggests that the altered cytopathic effect did not result from the shortening of the genome and possible consequential effects on the transcription or translation of the following attachment and polymerase protein genes.
2009
Proceedings of VI International Symposium on Avian Corona and Pneumovirus and Complicating Pathogens
9783835954847
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2372439
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