A study was performed to evaluate oxytetracycline (OTC) residue depletion in muscle, liver and kidney of turkeys receiving a 3-day administration of OTC hydrochloride in drinking water at 400 mg/L. The clean up procedure of tissue samples was performed as reported by Farrington et al. [1] but a mini SPE column was adopted, which allowed a considerable reduction of time, solvents and hazardous waste, and a recovery greater than 80%. The separation and identification of OTC and its derivative was easily obtained using isocratic HPLC condition and C18 column. Very low concentrations of the 4¢-epimer were detected at all sampling times in the kidney, up to the third day of withdrawal in liver and only at day 1 in muscle. The sum of OTC and 4-epi-OTC residues were by far lower than the MRL values after 3 days in all tissues, however, a safer withdrawal time, calculated according to the EU recommendation should be set at 5 days.

Depletion of oxytetracycline and its 4'-epimer in turkeys

CAPOLONGO, FRANCESCA;DE LIGUORO, MARCO;MONTESISSA, CLARA
2000

Abstract

A study was performed to evaluate oxytetracycline (OTC) residue depletion in muscle, liver and kidney of turkeys receiving a 3-day administration of OTC hydrochloride in drinking water at 400 mg/L. The clean up procedure of tissue samples was performed as reported by Farrington et al. [1] but a mini SPE column was adopted, which allowed a considerable reduction of time, solvents and hazardous waste, and a recovery greater than 80%. The separation and identification of OTC and its derivative was easily obtained using isocratic HPLC condition and C18 column. Very low concentrations of the 4¢-epimer were detected at all sampling times in the kidney, up to the third day of withdrawal in liver and only at day 1 in muscle. The sum of OTC and 4-epi-OTC residues were by far lower than the MRL values after 3 days in all tissues, however, a safer withdrawal time, calculated according to the EU recommendation should be set at 5 days.
Proceedings of 8th International Congress EAVPT, Jerusalem
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2431783
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