Estrogenic potency of 17ß-estradiol (E2) was evaluated in both sexually undifferentiated (resting phase) and differentiated (pre-spawning phase) clams (Tapes philippinarum) by exposing them for 7 and 14 days to various E2 concentrations (0, 0+acetone, 5, 25, 50, 100 and 1000 ng/L). Vitellogenin (Vg)-like protein levels were then evaluated in both haemolymph and digestive glands. In the resting phase, increased Vg-like protein levels were observed in haemolymph from clams exposed for 7 and 14 days to E2. Interestingly, significant decreases in Vg-like protein content were recorded in haemolymph from females exposed in the pre-spawning phase for 7 days to 50, 100, and 1000 ng E2/L. Conversely, 14 days E2 exposure was shown to increase Vg-like protein levels in haemolymph from both females (at 50 ng E2/L) and males (at 100 ng E2/L). Results obtained are difficult to explain, and suggest that further studies are needed to elucidate the role of E2 in promoting Vg induction in Tapes philippinarum.

Is vitellogenin-like protein induction responsive to 17-b estradiol? The clam Tapes philippinarum as a case study.

MATOZZO, VALERIO;MARIN, MARIA
2007

Abstract

Estrogenic potency of 17ß-estradiol (E2) was evaluated in both sexually undifferentiated (resting phase) and differentiated (pre-spawning phase) clams (Tapes philippinarum) by exposing them for 7 and 14 days to various E2 concentrations (0, 0+acetone, 5, 25, 50, 100 and 1000 ng/L). Vitellogenin (Vg)-like protein levels were then evaluated in both haemolymph and digestive glands. In the resting phase, increased Vg-like protein levels were observed in haemolymph from clams exposed for 7 and 14 days to E2. Interestingly, significant decreases in Vg-like protein content were recorded in haemolymph from females exposed in the pre-spawning phase for 7 days to 50, 100, and 1000 ng E2/L. Conversely, 14 days E2 exposure was shown to increase Vg-like protein levels in haemolymph from both females (at 50 ng E2/L) and males (at 100 ng E2/L). Results obtained are difficult to explain, and suggest that further studies are needed to elucidate the role of E2 in promoting Vg induction in Tapes philippinarum.
2007
PROCEEDINGS OF SECOTOX CONFERENCE AND THE INTERNATIONAL CONFERENCE ON ENVIRONMENTAL MANAGEMENT ENGINEERING, PLANNING AND ECONOMICS
9789608909083
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2467841
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