We have evaluated the reliability of the new fluorescence energy transfer immunoassay (FETI) for determining concentrations of digoxin in serum. The method demonstrated good stability of the standard curve and satisfactory precision in both within- and between-run assays. We have also found a significant corelation betwee FETI and two RIA methods (r = 0.97). Furthermore, the association between results by FETI and by EMIT method (a conventional enzyme immunoassay) was a linear one. Finally, the assay encountered no significant interference from endogenous substances (hemoglobin, bilirubin, and triglycerides).
Fluorescence energy transfer immunoassay of digoxin in serum.
PLEBANI, MARIO;
1985
Abstract
We have evaluated the reliability of the new fluorescence energy transfer immunoassay (FETI) for determining concentrations of digoxin in serum. The method demonstrated good stability of the standard curve and satisfactory precision in both within- and between-run assays. We have also found a significant corelation betwee FETI and two RIA methods (r = 0.97). Furthermore, the association between results by FETI and by EMIT method (a conventional enzyme immunoassay) was a linear one. Finally, the assay encountered no significant interference from endogenous substances (hemoglobin, bilirubin, and triglycerides).File in questo prodotto:
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