INTRODUCTION
 By
taking
advantage
of
reporter
fish
lines
generated
in
the
frame
of the
ZF‐HEALTH
project,
Partner
12
has
started
a
methodical
application of
these
biosensors
to
analyze
signaling
pathways
activation
during zebrafish
organ
formation.

 As
a
pilot
study,
we
have
focused
our
attention
on
the
zebrafish
eye, taking
into
consideration
a
series
of
key
properties
of
this
organ, among
which:
 ‐
early
formation
during
embryonic
development;
 ‐
optical
accessibility
due
to
its
superficial
position
and
relatively
large size
compared
to
other
embryonic
structures;
 ‐
high
evolutionary
conservation
of
its
components,
both
in
the anterior
and
posterior
segments
(e.g.
lens
structure;
retinal
cell
types);
 ‐ available
information
on
some
signaling
mechanisms,
derived
from previous
zebrafish
studies
or
different
model
systems.
 METHODOLOGY As
a
first
step,
we
have
selected
four
key
developmental
time points
for
our
analysis:
early
somitogenesis
(optic
lobe
evagination, lens
induction);
1
dpf
(lens
and
retina
both detectable);
2
dpf
(retinal
cell
type
formation);
3
dpf
(retinal
layer
formation,
visual
 function).

 For
each
time
points,
we
have
performed
both
in
vivo
and
post mortem
confocal
documentation
of
reporter
activity,
by
imaging fluorescent
protein
distribution
or
reporter
messenger
localization (either
alone
or
in
combination
with
ocular
markers).
 FUTURE PERSPECTIVES We
are
currently
completing this
general
picture
by
analyzing more
advanced
developmental
 stages,
as
well
as
including
two additional
pathway
reporters, Stat3
and
Glucocorticoid
 Receptor,
more
recently generated.
To
evaluate
roles and
epistatic
relationships
of
 signaling
pathways
activated
in the
same
ocular
district,
we
will perform
reporter
inter-crosses and
functional
activation/inactivation
of
each
involved molecular
cascade.


Application of zebrafish reporter lines for eye tissues analysis

TISO, NATASCIA;R. Dahm;MORO, ENRICO;VETTORI, ANDREA;ARGENTON, FRANCESCO
2012

Abstract

INTRODUCTION
 By
taking
advantage
of
reporter
fish
lines
generated
in
the
frame
of the
ZF‐HEALTH
project,
Partner
12
has
started
a
methodical
application of
these
biosensors
to
analyze
signaling
pathways
activation
during zebrafish
organ
formation.

 As
a
pilot
study,
we
have
focused
our
attention
on
the
zebrafish
eye, taking
into
consideration
a
series
of
key
properties
of
this
organ, among
which:
 ‐
early
formation
during
embryonic
development;
 ‐
optical
accessibility
due
to
its
superficial
position
and
relatively
large size
compared
to
other
embryonic
structures;
 ‐
high
evolutionary
conservation
of
its
components,
both
in
the anterior
and
posterior
segments
(e.g.
lens
structure;
retinal
cell
types);
 ‐ available
information
on
some
signaling
mechanisms,
derived
from previous
zebrafish
studies
or
different
model
systems.
 METHODOLOGY As
a
first
step,
we
have
selected
four
key
developmental
time points
for
our
analysis:
early
somitogenesis
(optic
lobe
evagination, lens
induction);
1
dpf
(lens
and
retina
both detectable);
2
dpf
(retinal
cell
type
formation);
3
dpf
(retinal
layer
formation,
visual
 function).

 For
each
time
points,
we
have
performed
both
in
vivo
and
post mortem
confocal
documentation
of
reporter
activity,
by
imaging fluorescent
protein
distribution
or
reporter
messenger
localization (either
alone
or
in
combination
with
ocular
markers).
 FUTURE PERSPECTIVES We
are
currently
completing this
general
picture
by
analyzing more
advanced
developmental
 stages,
as
well
as
including
two additional
pathway
reporters, Stat3
and
Glucocorticoid
 Receptor,
more
recently generated.
To
evaluate
roles and
epistatic
relationships
of
 signaling
pathways
activated
in the
same
ocular
district,
we
will perform
reporter
inter-crosses and
functional
activation/inactivation
of
each
involved molecular
cascade.

2012
ZF-HEALTH Meeting 2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2526297
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