Increased Neu presence has been reported in COPD, particularly in sputum and BAL from mild/moderate patients and in bronchial biopsies from severe diseased patients. CXC and CC chemokines and their receptors are thought to play a role in Neu chemoattraction and activation. Furthermore, increased tissutal neutrophilia may be related also to increased adhesion to submucosal collagens in the submucosa. We investigated the expression of CXCL7, CCL5, CD44 and CD11b in bronchial biopsies from subjects with severe COPD (n=13), mild/moderate COPD (n=12), and from control smokers (Ctr-S) (n=12) and control non smokers (Ctr-NS) (n=11). Immunopositivity was quantified as number of cells+/mm2 in the submucosa. Severe COPD and mild/moderate COPD had higher CXCL7+ in the submucosa (38(0–83) and 74(0–129)) compared to Ctr-NS (14(0–32)) but did not differ from Ctr-S (24(0–136). Severe COPD also had higher CCL5+ in the submucosa (281(75–839) compared to Ctr-NS (77(26–203)). No differences were observed in the total cell count of CD44 and CD11b receptors in the four groups examined. Double staining of Neu coexpressing CD44 and CD11b showed increased percentages of CD44+Neu+ and CD11b+Neu+ cells in severe COPD (48(39–54) and 47(38–52)) compared to Ctr-S (13(9–20) and 17(10–21)). These data show that increased presence of chemotactic factors for Neu and increased Neu adhesiveness may play a role in sustaining neutrophilia in patients with severe COPD.
Increased neutrophil (Neu) adhesion in bronchial biopsies from patients with severe COPD
BRUN, PAOLA;
2008
Abstract
Increased Neu presence has been reported in COPD, particularly in sputum and BAL from mild/moderate patients and in bronchial biopsies from severe diseased patients. CXC and CC chemokines and their receptors are thought to play a role in Neu chemoattraction and activation. Furthermore, increased tissutal neutrophilia may be related also to increased adhesion to submucosal collagens in the submucosa. We investigated the expression of CXCL7, CCL5, CD44 and CD11b in bronchial biopsies from subjects with severe COPD (n=13), mild/moderate COPD (n=12), and from control smokers (Ctr-S) (n=12) and control non smokers (Ctr-NS) (n=11). Immunopositivity was quantified as number of cells+/mm2 in the submucosa. Severe COPD and mild/moderate COPD had higher CXCL7+ in the submucosa (38(0–83) and 74(0–129)) compared to Ctr-NS (14(0–32)) but did not differ from Ctr-S (24(0–136). Severe COPD also had higher CCL5+ in the submucosa (281(75–839) compared to Ctr-NS (77(26–203)). No differences were observed in the total cell count of CD44 and CD11b receptors in the four groups examined. Double staining of Neu coexpressing CD44 and CD11b showed increased percentages of CD44+Neu+ and CD11b+Neu+ cells in severe COPD (48(39–54) and 47(38–52)) compared to Ctr-S (13(9–20) and 17(10–21)). These data show that increased presence of chemotactic factors for Neu and increased Neu adhesiveness may play a role in sustaining neutrophilia in patients with severe COPD.Pubblicazioni consigliate
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