Efecto de la adición en el pienso de mananoligosacáridos y β-glucanos de levaduras sobre la microbiota intestinal de gazapos

This work aimed to study the response of the ileal and caecal microbiota after dietary supplementation with MOS (Actigen, Alltech) and β-glucans. Sixty four weaning rabbits were randomly assigned to 4 experimental diets and fed ad libitum for 13 days as following: a control diet (C), G2 and M2 diets obtained supplementing C diet with 200 mg/kg of β-glucans or 800 mg/kg of MOS, respectively and MG diet was supplemented with 100 mg/kg of β-glucans + 400 mg/kg of MOS. The microbiota of ileum and caecum digesta was analyzed using TRFLP technique. Animals fed G2 and M2 decrease the biodiversity in ileum (P<0.010), but did not affect the biodiversity of the caecal microbiota. MG diet led to a microbiota distant from others diets both in ileum and caecum, especially from control group (R=0.95, R=0.69 respectively; P<0.001). Similarity analysis and diversity calculation provided that microbiota changes caused by the additives have been most important in ileum than in caecum. In conclusion, the MOS and the β-glucans cause changes in ileal and caecal microbiota of young rabbits after weaning. The mixture of both seems to have a distant effect than the individual components.