New modified recombinant human tyrosinase, useful for identifying tyrosinase modulators used for treating skin diseases

NOVELTY - An isolated protein having the amino acid sequence of SEQ ID NO. 5 is new. USE - The isolated (glycosylated) protein is useful for identifying tyrosinase modulators, preferably tyrosinase inhibitors (claimed). The tyrosinase inhibitor is useful for treating skin diseases. ADVANTAGE - A method for producing recombinant active human tyrosinase in amounts, which would allow the screening of efficient and specific inhibitors and selective drugs for the treatment of pathologies in which the enzyme is over or under expressed, is provided. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are: (1) an isolated polynucleotide encoding the protein above; and (2) a method of screening for modulators of a tyrosinase polypeptide by (a) incubating the isolated glycosylated protein with a screening compound, and (b) detecting the binding between the isolated glycosylated protein and the screening compound.