In this work the injection molding of micro-structured polystyrene surfaces and their application in stimulating growth of osteoblastic MC3T3-E1 cells is described. The experimental results obtained from micro-pillared surfaces show that among the physicochemical parameters of the biomaterial surface, such as surface energy, surface charges or chemical composition, micro-topography is one of the most crucial factor influencing cell adhesion and proliferation. Furthermore, the molding process parameters have been optimized to increase the degree of micro pillars replication and to induce cell growth. MTS results showed a better correlation of cell proliferation with micro pillars diameter and spacing than with their height or aspect ratio.

Development of osteoinductive micro-structured polymer surfaces for mesenchymal stem cells differentiation

LUCCHETTA, GIOVANNI;SORGATO, MARCO;ZANCHETTA, ERIKA;BRUSATIN, GIOVANNA
2014

Abstract

In this work the injection molding of micro-structured polystyrene surfaces and their application in stimulating growth of osteoblastic MC3T3-E1 cells is described. The experimental results obtained from micro-pillared surfaces show that among the physicochemical parameters of the biomaterial surface, such as surface energy, surface charges or chemical composition, micro-topography is one of the most crucial factor influencing cell adhesion and proliferation. Furthermore, the molding process parameters have been optimized to increase the degree of micro pillars replication and to induce cell growth. MTS results showed a better correlation of cell proliferation with micro pillars diameter and spacing than with their height or aspect ratio.
Proceedings of the 6th International Conference on Polymers and Moulds Innovations
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11577/3162892
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