Arbuscular mycorrhizal fungi (AMF) are plant growth promoters known to ameliorate plant water uptake and nutrient availability in wheat. In this work, two cultivars of Triticum spp., i.e. a bread (Chinese Spring) and a durum (Mongibello) wheat grown under drought stress and inoculated or not by AMF (Glomus mosseae), were evaluated through a shotgun proteomic approach. The beneficial AMF symbiosis was confirmed by measuring morphological and physiological traits, increasing aboveground biomass in both wheat genotypes. Overall, 50 and 66 statistically differential proteins could be identified in bread and durum wheat cultivars, respectively. The proteomic results confirmed a genotype dependent response to AMF against drought. The findings highlighted a modulation of proteins related to sugar metabolism as well as proteins related to plant stress responses. The interactome analysis supported an involvement of cell wall metabolism and rearrangement, and a possible role of ubiquitin in mycorrhizal roots. In order to gain a deeper insight into molecular processes involved in wheat response to drought following AMF colonization, an UHPLC- ESI/QTOF-MS metabolomic analysis was then carried out. Multivariate chemometrics allowed highlighting differential metabolites. Among the differential metabolic compounds we identified lipids as a new carbon source and as precursors of brassinosteroid biosynthetic pathway. Although useful insights were provided from both proteomics and metabolomics, a cultivar-dependent response was observed.

Proteomic and metabolomic insight into two wheat response to drought following AMF inoculation

Paolo Carletti;
2018

Abstract

Arbuscular mycorrhizal fungi (AMF) are plant growth promoters known to ameliorate plant water uptake and nutrient availability in wheat. In this work, two cultivars of Triticum spp., i.e. a bread (Chinese Spring) and a durum (Mongibello) wheat grown under drought stress and inoculated or not by AMF (Glomus mosseae), were evaluated through a shotgun proteomic approach. The beneficial AMF symbiosis was confirmed by measuring morphological and physiological traits, increasing aboveground biomass in both wheat genotypes. Overall, 50 and 66 statistically differential proteins could be identified in bread and durum wheat cultivars, respectively. The proteomic results confirmed a genotype dependent response to AMF against drought. The findings highlighted a modulation of proteins related to sugar metabolism as well as proteins related to plant stress responses. The interactome analysis supported an involvement of cell wall metabolism and rearrangement, and a possible role of ubiquitin in mycorrhizal roots. In order to gain a deeper insight into molecular processes involved in wheat response to drought following AMF colonization, an UHPLC- ESI/QTOF-MS metabolomic analysis was then carried out. Multivariate chemometrics allowed highlighting differential metabolites. Among the differential metabolic compounds we identified lipids as a new carbon source and as precursors of brassinosteroid biosynthetic pathway. Although useful insights were provided from both proteomics and metabolomics, a cultivar-dependent response was observed.
3rd INPPO World Congress Book of Abstracts
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11577/3279667
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