Celi suspension cultures represent a widely used experimental tool suitable to perform a variety of structural and physiological studies in a more simplified system compared to the organism in toto. In this chapter we describe the methods routinely used in our laboratory to establish and maintain Arabidopsis photosynthetic and heterotrophic cell suspension cultures, containing either chloroplasts or amyloplasts, respectively. The use of these in vitro systems may allow to obtain insights into the unique features of chloroplasts versus non-green plastids, as well as their integration in the structural and metabolic compartmentalization of the plant cell.

Arabidopsis photosynthetic and heterotrophic cell suspension cultures

Enrico Cortese;Luca Carraretto;Barbara Baldan;Lorella Navazio
2021

Abstract

Celi suspension cultures represent a widely used experimental tool suitable to perform a variety of structural and physiological studies in a more simplified system compared to the organism in toto. In this chapter we describe the methods routinely used in our laboratory to establish and maintain Arabidopsis photosynthetic and heterotrophic cell suspension cultures, containing either chloroplasts or amyloplasts, respectively. The use of these in vitro systems may allow to obtain insights into the unique features of chloroplasts versus non-green plastids, as well as their integration in the structural and metabolic compartmentalization of the plant cell.
2021
Arabidopsis Protocols 4th Edition - Methods in Molecular Biology
978-1-0716-0879-1
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/3338567
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