Protein tyrosine kinases (TKs) play a crucial role in human physiology and their abnormal function, due to protein modifications, is correlated with several diseases. The clear need to understand the mechanisms and to antagonize the activities of aberrant catalytic domains of TKs (KD) fired up this thesis. Through the chapters, evaluations about sequence, structure, dynamics and ligand binding of these proteins are made using several computational tools. Firstly, the essential structural elements for molecules to bind at the ATP-binding site are defined. Then, the dynamical behavior of different KDs is classified based on the rearrangement of 5 clusters of residues. In particular, a pool of residues, found at the interface of the two lobes, appears to be important for the protein conformation and motion. Finally, the investigation about the activation mechanism of oncogenic Flt3 is reported and a secondary structure element suggested as the first driving force for conformational changes.
Exploring Structure and Plasticity of Tyrosine Kinase Domains for Drug Discovery(2007).
Exploring Structure and Plasticity of Tyrosine Kinase Domains for Drug Discovery
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2007
Abstract
Protein tyrosine kinases (TKs) play a crucial role in human physiology and their abnormal function, due to protein modifications, is correlated with several diseases. The clear need to understand the mechanisms and to antagonize the activities of aberrant catalytic domains of TKs (KD) fired up this thesis. Through the chapters, evaluations about sequence, structure, dynamics and ligand binding of these proteins are made using several computational tools. Firstly, the essential structural elements for molecules to bind at the ATP-binding site are defined. Then, the dynamical behavior of different KDs is classified based on the rearrangement of 5 clusters of residues. In particular, a pool of residues, found at the interface of the two lobes, appears to be important for the protein conformation and motion. Finally, the investigation about the activation mechanism of oncogenic Flt3 is reported and a secondary structure element suggested as the first driving force for conformational changes.File | Dimensione | Formato | |
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