Biological tissue substitutes need to be appropriately stored in order to provide ready-to-use solutions for patients with immediate necessity. In this preliminary study, different native porcine tissues (i.e., pericardium, ureter, descending aorta, and small intestinal submucosa) were subjected to cryopreservation at -80°C with a specific solution based on the use of 10% DMSO with the aim to evaluate the effects on ECM composition by means of histological analysis and biomechanical tests (i.e., tensile and stress-relaxation tests). Morphologically, all tissues appeared microscopically well preserved; from the biomechanical point of view, some tissue-dependent changes were detected.
Cryopreservation effects on biological tissues: histological and mechanical assessments
Casarin M.;Todesco M.;Fontanella C. G.;Morlacco A.;Dal Moro F.;Bagno A.
2023
Abstract
Biological tissue substitutes need to be appropriately stored in order to provide ready-to-use solutions for patients with immediate necessity. In this preliminary study, different native porcine tissues (i.e., pericardium, ureter, descending aorta, and small intestinal submucosa) were subjected to cryopreservation at -80°C with a specific solution based on the use of 10% DMSO with the aim to evaluate the effects on ECM composition by means of histological analysis and biomechanical tests (i.e., tensile and stress-relaxation tests). Morphologically, all tissues appeared microscopically well preserved; from the biomechanical point of view, some tissue-dependent changes were detected.
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