Biological evaluation in vitro and in vivo of hybrid membrane to assess biomedical application

Currently the main challenge of tissue engineering is the realization of materials that can replace tissues and organs and perfectly integrate with the host organism ensuring the proper functionality. Hybrid Membranes (HYMEs) were produced by coupling a decellularized tissue (porcine pericardium) with a polymeric material (polycarbonate urethane). HYMEs biocompatibility was assessed in vitro by direct contact cells seeding and in vivo by implantation in the rat model. In vitro no cytotoxicity was observed and cells growth was demonstrated until 7 days. In vivo HYMEs appeared better integrated with the host tissue than the polymer alone (control) after 4 weeks from implantation. Moreover, the polymer was not affected by degradation.