Impact of the light-induced stress on the chemico-physical and biological properties of two monoclonal antibodies, bevacizumab (Avastin®) and durvalumab (Imfinzi®)

INFLUENCE OF A THERAPEUTIC MONOCLONAL ANTIBODY FORMULATION BEFORE AND AFTER IRRADIATION ON THE SURFACE TENSIOMETRY PROPERTIES OF BLOOD DROPLETS MEASURED BY THE CONTACT ANGLE METHOD During storage, transport, dilution and intravenous (i.v.) administration, mAbs are exposed to different stressors which can compromise their efficacy and safety. Light can induce concentration-dependent aggregation, leading to a measurable reduction in the drug's ability to bind its target. The surface tensiometry properties of monoclonal antibodies (mAbs) were recently characterized using the Solid-like Methodology (SLM) [1] applying the Contact Angle Method (CA) [2]. [1] MIOLO G, DE DIANA E, POLVERINO DE LAURETO P, REALDON N, ROSSI S, ROSSI D. J PHARM SCI, 114 (7):1-12, 2025, [2] Etzler FM. Mittal (eds) Advances in Contact Angle Wettability&Adhesion, 13: 229-329. Scrivener Publishing LLC, 2018. [3] Rossi D. Balneo and PRM Research Journal, 14(4):1-17, 2023, [4] Rossi D, Rossi S, Realdon N. Mittal (ed) Advances and Contact Angles Wettability&Adhesion, 4:145-178. Scrivener Publishing, Beverly, MA, 2019. [5] Rossi D,Vettorato E, Franceschinis E. Grotto A. Favaro A. Realdon N. Progress in Adhesion & Adhesives IN PRESS, 2026.