Synchrotron Radiation FTIR microspectroscopy (SR-μFTIR) is a technique that allows to explore biochemical status of biological specimen with cellular and sub cellular spatial resolution. However, the possibility to follow in real-time dynamic evolutions of biosystems is nowadays limited by the lack of microfluidic devices built on IR-transparent materials. We present the fabrication of and first measurements in novel microfluidic devices, fabricated on CaF2 windows. Test measurements were carried on Jurkat T cells at different extents of mechanical deformation, showing an increasing in plasma membrane disorder and cellular protein rearrangement as a function of compression. © 2009 CBMS.
Single living cell biochemical rearrangements investigated by infrared micro-spectroscopy in microfluidic devices
Grenci G.;
2009
Abstract
Synchrotron Radiation FTIR microspectroscopy (SR-μFTIR) is a technique that allows to explore biochemical status of biological specimen with cellular and sub cellular spatial resolution. However, the possibility to follow in real-time dynamic evolutions of biosystems is nowadays limited by the lack of microfluidic devices built on IR-transparent materials. We present the fabrication of and first measurements in novel microfluidic devices, fabricated on CaF2 windows. Test measurements were carried on Jurkat T cells at different extents of mechanical deformation, showing an increasing in plasma membrane disorder and cellular protein rearrangement as a function of compression. © 2009 CBMS.
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