The recent developement of stain-clearing techniques allowed an increase in the information concerning cytological mechanisms responsible for 2n egg formation. Using these methodologies, our research group has already characterized several diploid alfalfa mutants producing 2n eggs at high frequency. Moreover, it was verified that stain-clearing techniques can be efficiently applied also to the cytological analysis of microsporogenesis and therefore they will be useful for a quick cytological analysis of mutants producing both 2n pollen and 2n eggs. In the present paper, our results, together with the potential applications of stain-clearing techniques in advanced breeding programs based on the use of 2n gametes, are discussed.
L'osservazione citologica ed istologica come mezzo d'indagine nello studio dei sistemi riproduttivi.
BARCACCIA, GIANNI;
1997
Abstract
The recent developement of stain-clearing techniques allowed an increase in the information concerning cytological mechanisms responsible for 2n egg formation. Using these methodologies, our research group has already characterized several diploid alfalfa mutants producing 2n eggs at high frequency. Moreover, it was verified that stain-clearing techniques can be efficiently applied also to the cytological analysis of microsporogenesis and therefore they will be useful for a quick cytological analysis of mutants producing both 2n pollen and 2n eggs. In the present paper, our results, together with the potential applications of stain-clearing techniques in advanced breeding programs based on the use of 2n gametes, are discussed.Pubblicazioni consigliate
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