Analytical validation of a general protocol for the preparation of dose-controlled solution to be proposed in aluminium toxicology

Aluminium(III) solutions suitable fbr careful toxicological investigation both in vivo and in vitro, are prepared from AlCl3 . 6H(2)O and from neutral metal complexes, Al(lactate)(3), Al-2(citrate)(2)(H2O)(6), Al(acetylacetonate)(3) and Al(maltolate)(3), by dissolving the pure compounds in TRIS-HCI buffer solutions. The metal concentrations in the supernatant liquids are determined spectrophotometrically under reproducible conditions. Al(acetylacetonate)(3) and Al(maltolate)(3) are detected as molecular entities by using H-1 NMR spectroscopy down to concentrations of 200 and 100 mu mol 1(-1), respectively.