The UVA photobinding of 6,4,4'-trimethylangelicin to a single stranded DNA (PEMBL-derived plasmid, 3873 bp) was studied by means of sequencing PAGE electrophoresis. The preferential photobinding of TMA was demonstrated at TC sites. Indeed, the Klenow fragment stopped one nucleotide before both TMA- thymine and cytosine photoadducts.

Determination of base specificity in 6,4,4'-trimethylangelicin photobinding to single-stranded DNA

MIOLO, GIORGIA;SCARPA, MAURIZIO;DALL'ACQUA, FRANCESCO;ZACCHELLO, FRANCO
1989

Abstract

The UVA photobinding of 6,4,4'-trimethylangelicin to a single stranded DNA (PEMBL-derived plasmid, 3873 bp) was studied by means of sequencing PAGE electrophoresis. The preferential photobinding of TMA was demonstrated at TC sites. Indeed, the Klenow fragment stopped one nucleotide before both TMA- thymine and cytosine photoadducts.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11577/128627
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