We have determined the effect of parental cell concentration at the time of fusion with polyethylene glycol on the frequency of viable somatic hybrids and of polykaryocytes of different multiplicity, between Chinese hamster (line Wg3) and mouse (line 3TP) fibroblasts. Fusion appeared to be dictated by the random encounter of cells only at low cell concentrations. However, no evidence for species-specific restriction of fusion was obtained at any cell concentration.
Hybridization frequencies of chinese hamster and mouse fibroblasts fused with polyethylene glycol at different cell densities.
LANFRANCHI, GEROLAMO;
1981
Abstract
We have determined the effect of parental cell concentration at the time of fusion with polyethylene glycol on the frequency of viable somatic hybrids and of polykaryocytes of different multiplicity, between Chinese hamster (line Wg3) and mouse (line 3TP) fibroblasts. Fusion appeared to be dictated by the random encounter of cells only at low cell concentrations. However, no evidence for species-specific restriction of fusion was obtained at any cell concentration.File in questo prodotto:
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