Selective binding of TAR RNA by a tat-derived beta-peptide

The interaction between the HIV-1 Tat protein and the TAR RNA element in the nascent viral genomic transcript is required for viral replication. An 11-residue beta-peptide (1), an all-beta homologue of the Arg-rich region Tat 47-57, binds TAR RNA with K(d) = 29 +/- 4 nM. A control beta-peptide (2) in which all Arg side chains are replaced by Lys side chains shows increased affinity but decreased specificity for wild-type vs bulge-deleted TAR RNA, as do the alpha-peptide analogues of 1 and 2.

Titolo:	Selective binding of TAR RNA by a tat-derived beta-peptide
Autori:	GELMAN MA RICHTER, SARA CAO H UMEZAWA N GELLMAN SH RANA TM mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2003
Rivista:	ORGANIC LETTERS
Abstract:	The interaction between the HIV-1 Tat protein and the TAR RNA element in the nascent viral genomic transcript is required for viral replication. An 11-residue beta-peptide (1), an all-beta homologue of the Arg-rich region Tat 47-57, binds TAR RNA with K(d) = 29 +/- 4 nM. A control beta-peptide (2) in which all Arg side chains are replaced by Lys side chains shows increased affinity but decreased specificity for wild-type vs bulge-deleted TAR RNA, as do the alpha-peptide analogues of 1 and 2.
Handle:	http://hdl.handle.net/11577/151238
Appare nelle tipologie:	01.01 - Articolo in rivista

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