The nucleotide sequence of two segments of the mtDNA molecule of different species of Drosophila containing a portion of the large rRNA (16S) and a portion of the small rRNA (23S) has been determined. The polymerase chain reaction (PCR) technique with ''universal'' primers has been used to amplify the segments for direct sequencing. The species analysed belong to four different subgroups of the melanogaster species group. The phylogenetic relationships of the species have been reconstructed by using the neighbour-joining algorithm and the significance of the inferred phylogeny tested by bootstrap method.
EVOLUTION OF THE MITOCHONDRIAL RIBOSOMAL-RNA IN THE ORIENTAL SPECIES SUBGROUPS OF DROSOPHILA
NIGRO, LOREDANA;GRAPPUTO, ALESSANDRO
1993
Abstract
The nucleotide sequence of two segments of the mtDNA molecule of different species of Drosophila containing a portion of the large rRNA (16S) and a portion of the small rRNA (23S) has been determined. The polymerase chain reaction (PCR) technique with ''universal'' primers has been used to amplify the segments for direct sequencing. The species analysed belong to four different subgroups of the melanogaster species group. The phylogenetic relationships of the species have been reconstructed by using the neighbour-joining algorithm and the significance of the inferred phylogeny tested by bootstrap method.File in questo prodotto:
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