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EnglishWe have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced.
| Titolo: | Comparison of MS/MS methods for protein identification from 2D-PAGE |
| Autori: | |
| Data di pubblicazione: | 2006 |
| Rivista: | |
| Abstract: | We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced. |
| Handle: | http://hdl.handle.net/11577/153562 |
| Appare nelle tipologie: | 01.01 - Articolo in rivista |
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