Comparison of MS/MS methods for protein identification from 2D-PAGE

Arrigoni, Giorgio; Fernandez, C; Holm, C; Scigelova, M; James, P.

doi:10.1021/pr0601281

We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced.

Titolo:	Comparison of MS/MS methods for protein identification from 2D-PAGE
Autori:	ARRIGONI, GIORGIO FERNANDEZ C HOLM C SCIGELOVA M JAMES P. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2006
Rivista:	JOURNAL OF PROTEOME RESEARCH
Abstract:	We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced.
Handle:	http://hdl.handle.net/11577/153562
Appare nelle tipologie:	01.01 - Articolo in rivista

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