We show that beta(2)-Microglobulin solutions in physiological conditions contain a tiny fraction of aggregates, which can hardly be filtered out and tend to re-form spontaneously. At physiological pH the fractional amount and size distribution of the latter aggregates do not depend on temperature. Conversely, in the pH range typical of the peri-articular tissue acidosis that often occurs in hemodialysis, temperature increase leads to fast and irreversible growth of the aggregates. Quite similar, but strongly enhanced aggregation effects can be induced even in physiological conditions by adding a very small amount of Delta N6, a naturally occurring truncated isoform of beta(2)-m known to promote fibrillogenesis.

Micro-heterogeneity and aggregation in beta(2)-microglobulin solutions: effects of temperature, pH, and conformational variant addition

PIERNO, MATTEO AMBROGIO PAOLO;
2006

Abstract

We show that beta(2)-Microglobulin solutions in physiological conditions contain a tiny fraction of aggregates, which can hardly be filtered out and tend to re-form spontaneously. At physiological pH the fractional amount and size distribution of the latter aggregates do not depend on temperature. Conversely, in the pH range typical of the peri-articular tissue acidosis that often occurs in hemodialysis, temperature increase leads to fast and irreversible growth of the aggregates. Quite similar, but strongly enhanced aggregation effects can be induced even in physiological conditions by adding a very small amount of Delta N6, a naturally occurring truncated isoform of beta(2)-m known to promote fibrillogenesis.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11577/155181
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