Percutaneous neuromuscular electrical stimulation (NMES) has been sucessfully used in rehabilitation programs in geriatric, cardiovascular and orthopedic medicine. The major advantage of NMES over voluntary training is the lower risk of traumatic lesions and falls in subjects with high degree of sarcopenia and the possibility of a very standardized training. Whereas the impact of NMES on skeletal muscle mass and function has been described in detail, no information are available on the molecular mechanisms underlying such adaptations. In this study, ten, young (18–35 years of age), healthy, male subjects were subjected to 24, 18-min sessions of isometric (bilateral) NMES of the quadriceps muscle over a period of 8 weeks with 3 sessions per week. Needles biopsies were taken from the vastus lateralis muscles pre- and post-training. Maximum voluntary contraction (MVC) and neural activation were found to be significantly higher post-training (?20 and ?9%, respectively). MHC isoform distribution showed a significant shift from MHC-2X towards MHC-2A and MHC-1, i.e. a fast to slow transition. Real-time PCR analysis of changes in MHC expression showed the same pattern. Fluorescently stained proteomic maps showing *600 spots were obtained pre- and post-training and differentially expressed proteins were identified.

Human skeletal muscle adaptations to neuromuscolar electrical stimulation training: a proteomic analysis

MACCATROZZO, LISA;
2008

Abstract

Percutaneous neuromuscular electrical stimulation (NMES) has been sucessfully used in rehabilitation programs in geriatric, cardiovascular and orthopedic medicine. The major advantage of NMES over voluntary training is the lower risk of traumatic lesions and falls in subjects with high degree of sarcopenia and the possibility of a very standardized training. Whereas the impact of NMES on skeletal muscle mass and function has been described in detail, no information are available on the molecular mechanisms underlying such adaptations. In this study, ten, young (18–35 years of age), healthy, male subjects were subjected to 24, 18-min sessions of isometric (bilateral) NMES of the quadriceps muscle over a period of 8 weeks with 3 sessions per week. Needles biopsies were taken from the vastus lateralis muscles pre- and post-training. Maximum voluntary contraction (MVC) and neural activation were found to be significantly higher post-training (?20 and ?9%, respectively). MHC isoform distribution showed a significant shift from MHC-2X towards MHC-2A and MHC-1, i.e. a fast to slow transition. Real-time PCR analysis of changes in MHC expression showed the same pattern. Fluorescently stained proteomic maps showing *600 spots were obtained pre- and post-training and differentially expressed proteins were identified.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2273116
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