We synthesized by solution-phase methods three analogues, [L-Leu6-OMe], [L-(αMe)Leu3, L-Leu6-OMe], and [L-(αMe)Val4, L-Leu6-OMe] of halovir A. The [L-Leu6-OMe] analogue is known to be biologically equipotent to its naturally occurring, antiviral, lipopentapeptide amide parent compound. The preferred conformations of the L-(αMe)Leu- and L-(αMe)Val-containing analogues, with a potentially reinforced helicity, were compared with those of [L-Leu6-OMe] halovir A and the natural peptide itself by use of a combination of FT-IR absorption and NMR techniques. Measurements of the antiviral activities against herpes simplex virus type-1 (HSV-1) of halovir A and its three analogues were also carried out. Interestingly, the [L-(αMe)Val4, L-Leu6-OMe] analogue exhibits the most significant activity in reducing HSV-1 infectivity, notably higher than that of halovir A itself
Synthesis, conformation, and bioactivity of novel analogues of the antiviral lipopeptide halovir A
FORMAGGIO, FERNANDO;PEGGION, CRISTINA;TONIOLO, CLAUDIO
2006
Abstract
We synthesized by solution-phase methods three analogues, [L-Leu6-OMe], [L-(αMe)Leu3, L-Leu6-OMe], and [L-(αMe)Val4, L-Leu6-OMe] of halovir A. The [L-Leu6-OMe] analogue is known to be biologically equipotent to its naturally occurring, antiviral, lipopentapeptide amide parent compound. The preferred conformations of the L-(αMe)Leu- and L-(αMe)Val-containing analogues, with a potentially reinforced helicity, were compared with those of [L-Leu6-OMe] halovir A and the natural peptide itself by use of a combination of FT-IR absorption and NMR techniques. Measurements of the antiviral activities against herpes simplex virus type-1 (HSV-1) of halovir A and its three analogues were also carried out. Interestingly, the [L-(αMe)Val4, L-Leu6-OMe] analogue exhibits the most significant activity in reducing HSV-1 infectivity, notably higher than that of halovir A itselfPubblicazioni consigliate
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