IF(2003): 6.48 ISI: Bioch & Mol Biol The mitochondrial permeability transition pore and Bax have both been proposed to be involved in the release of pro-apoptotic factors from mitochondria in the "intrinsic" pathway of apoptosis. The permeability transition pore is widely thought to be a supramolecular complex including or interacting with Bax. Given the relevance of the permeability transition in vivo, we have verified whether Bax influences the formation and/or the properties of the Ca2+/P-i-induced permeability transition by using mitochondria isolated from isogenic human colon cancer bax(+/-) and bax(-/-) HCT116 cell lines. We used mitochondria isolated from both types of cells and from Bax(+) cells exposed to apoptotic stimuli, as well as Bax-less mitochondria into which exogenous Bax had been incorporated. All exhibited the same behavior and pharmacological profile in swelling and Ca2+-retention experiments. Mitochondria from a bax(-)/bak(-) cell line also underwent an analogous Ca2+/P-i-inducible swelling. This similarity indicates that Bax has no major role in regulating the Ca2+-induced mitochondrial permeability transition.

Bax does not directly participate in the Ca2+-induced permeability transition of isolated mitochondria

DE MARCHI, UMBERTO;SZABO', ILDIKO';TOMBOLA, FRANCESCO;
2004

Abstract

IF(2003): 6.48 ISI: Bioch & Mol Biol The mitochondrial permeability transition pore and Bax have both been proposed to be involved in the release of pro-apoptotic factors from mitochondria in the "intrinsic" pathway of apoptosis. The permeability transition pore is widely thought to be a supramolecular complex including or interacting with Bax. Given the relevance of the permeability transition in vivo, we have verified whether Bax influences the formation and/or the properties of the Ca2+/P-i-induced permeability transition by using mitochondria isolated from isogenic human colon cancer bax(+/-) and bax(-/-) HCT116 cell lines. We used mitochondria isolated from both types of cells and from Bax(+) cells exposed to apoptotic stimuli, as well as Bax-less mitochondria into which exogenous Bax had been incorporated. All exhibited the same behavior and pharmacological profile in swelling and Ca2+-retention experiments. Mitochondria from a bax(-)/bak(-) cell line also underwent an analogous Ca2+/P-i-inducible swelling. This similarity indicates that Bax has no major role in regulating the Ca2+-induced mitochondrial permeability transition.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2438404
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