Background: Parathyroid carcinoma (PC) is an uncommon cancer. Unfortunately, histopathological distinction between PC and parathyroid adenoma is still difficult and, moreover, the clinical outcome of patients varies widely. The aim of this study was to assess the usefulness of proliferating cell nuclear antigen labeling index (PCNA-LI), Ki-67 antigen, and tumor nuclear DNA index (DI) in patients with parathyroid carcinoma. Patients and Methods: Paraffin-embedded archivial tissue sections from 15 patients (11 men, 4 women, median age 65 years, range 30-68 years) with confirmed PC who died of the disease were reviewed. Specimens were stained by using streptavidinbiotin-peroxidase complex standard technique and monoclonal antibodies anti-PCNA and anti-Ki-67 (MIB-1). The DNA flow cytometry to determine DI was also used. Results: The greatest diameter (size) of the tumor measured by the pathologist was 29±9 mm. The mean survival of patients was 47±37 years (median 29 months, range 21-146). Diploid (DI=1) and aneuploid (DI>1) tumors were found in 4 and 11 cases, respectively. The median PCNA-LI was 13% (range 2-70%), and the median MIB-1 value was 11% (range 3-65%). The survivals of patients with diploid and aneuploid tumors were 74±58 months and 34±18 months (p=0.21), respectively. There was a linear relationship between PCNA-LI and MIB-1 (R=0.93, p<0.01), but no correlation between age and survival (R=0.08, p=0.78) was found. Moreover, there was no correlation between survival and DI (R=0.17, p=0.55), PCNA-LI (R=0.07, p=0.72), and MIB-1 (R=-0.05, p=0.86). Conclusions: PCNA-LI, MIB-1, and DI may be useful in confirming the diagnosis of malignancy, but they are of little value in predicting the clinical outcome of patients with PC.
Immunohistochemical markers PCNA-LI and Ki-67, and DNA index in patients with parathyroid carcinoma
LUMACHI, FRANCO;IACOBONE, MAURIZIO;
2005
Abstract
Background: Parathyroid carcinoma (PC) is an uncommon cancer. Unfortunately, histopathological distinction between PC and parathyroid adenoma is still difficult and, moreover, the clinical outcome of patients varies widely. The aim of this study was to assess the usefulness of proliferating cell nuclear antigen labeling index (PCNA-LI), Ki-67 antigen, and tumor nuclear DNA index (DI) in patients with parathyroid carcinoma. Patients and Methods: Paraffin-embedded archivial tissue sections from 15 patients (11 men, 4 women, median age 65 years, range 30-68 years) with confirmed PC who died of the disease were reviewed. Specimens were stained by using streptavidinbiotin-peroxidase complex standard technique and monoclonal antibodies anti-PCNA and anti-Ki-67 (MIB-1). The DNA flow cytometry to determine DI was also used. Results: The greatest diameter (size) of the tumor measured by the pathologist was 29±9 mm. The mean survival of patients was 47±37 years (median 29 months, range 21-146). Diploid (DI=1) and aneuploid (DI>1) tumors were found in 4 and 11 cases, respectively. The median PCNA-LI was 13% (range 2-70%), and the median MIB-1 value was 11% (range 3-65%). The survivals of patients with diploid and aneuploid tumors were 74±58 months and 34±18 months (p=0.21), respectively. There was a linear relationship between PCNA-LI and MIB-1 (R=0.93, p<0.01), but no correlation between age and survival (R=0.08, p=0.78) was found. Moreover, there was no correlation between survival and DI (R=0.17, p=0.55), PCNA-LI (R=0.07, p=0.72), and MIB-1 (R=-0.05, p=0.86). Conclusions: PCNA-LI, MIB-1, and DI may be useful in confirming the diagnosis of malignancy, but they are of little value in predicting the clinical outcome of patients with PC.Pubblicazioni consigliate
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