The association of 12a,14a-diazoniapentaphene dibromide (5) with DNA and its ability to photoinduce DNA damage was studied. Spectrophotometric and fluorimetric titrations show that the title compound (5) binds to DNA (K ≈ 5·105 M–1) with the highest affinity towards GC-rich regions. The highest affinity was observed for GC base pairs. Compound 5 binds to DNA primarily by intercalation as shown by LD spectroscopy and energy-transfer experiments. Moreover, efficient DNA damage was observed on UVA irradiation in the presence of 5. Preliminary experiments suggest that singlet oxygen may be involved in the photoinduced DNA damage.
DNA-binding and DNA-photocleavaging properties of 12a,14a-diazoniapentaphene
VIOLA G;VEDALDI, DANIELA ESTER;DALL'ACQUA, FRANCESCO
2004
Abstract
The association of 12a,14a-diazoniapentaphene dibromide (5) with DNA and its ability to photoinduce DNA damage was studied. Spectrophotometric and fluorimetric titrations show that the title compound (5) binds to DNA (K ≈ 5·105 M–1) with the highest affinity towards GC-rich regions. The highest affinity was observed for GC base pairs. Compound 5 binds to DNA primarily by intercalation as shown by LD spectroscopy and energy-transfer experiments. Moreover, efficient DNA damage was observed on UVA irradiation in the presence of 5. Preliminary experiments suggest that singlet oxygen may be involved in the photoinduced DNA damage.File in questo prodotto:
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