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EnglishTwo different models of hepatocyte transplantation were used, dividing the study into three groups: (1) intrasplenic hepatocyte transplantation (HcTx) and (2) intraperitoneal hepatocytes attached porcine autologous biomatrix transplantation (PBMHcTx). A standard collagenase technique was used to prepare porcine liver cells. The viability of harvested cells as determined by Trypan blue dye exclusion test was 90%. The hepatocytes were cryopreserved for 1 month using two-step technique5 and thawed at the time of transplantation (75% viability).
| Titolo: | Hepatocyte transplantation: an experimental study to treat acute liver failure in pigs |
| Autori: | |
| Data di pubblicazione: | 2001 |
| Rivista: | |
| Abstract: | Two different models of hepatocyte transplantation were used, dividing the study into three groups: (1) intrasplenic hepatocyte transplantation (HcTx) and (2) intraperitoneal hepatocytes attached porcine autologous biomatrix transplantation (PBMHcTx). A standard collagenase technique was used to prepare porcine liver cells. The viability of harvested cells as determined by Trypan blue dye exclusion test was 90%. The hepatocytes were cryopreserved for 1 month using two-step technique5 and thawed at the time of transplantation (75% viability). |
| Handle: | http://hdl.handle.net/11577/2454104 |
| Appare nelle tipologie: | 01.01 - Articolo in rivista |
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