Two different models of hepatocyte transplantation were used, dividing the study into three groups: (1) intrasplenic hepatocyte transplantation (HcTx) and (2) intraperitoneal hepatocytes attached porcine autologous biomatrix transplantation (PBMHcTx). A standard collagenase technique was used to prepare porcine liver cells. The viability of harvested cells as determined by Trypan blue dye exclusion test was 90%. The hepatocytes were cryopreserved for 1 month using two-step technique5 and thawed at the time of transplantation (75% viability).
Titolo: | Hepatocyte transplantation: an experimental study to treat acute liver failure in pigs | |
Autori: | ||
Data di pubblicazione: | 2001 | |
Rivista: | ||
Abstract: | Two different models of hepatocyte transplantation were used, dividing the study into three groups: (1) intrasplenic hepatocyte transplantation (HcTx) and (2) intraperitoneal hepatocytes attached porcine autologous biomatrix transplantation (PBMHcTx). A standard collagenase technique was used to prepare porcine liver cells. The viability of harvested cells as determined by Trypan blue dye exclusion test was 90%. The hepatocytes were cryopreserved for 1 month using two-step technique5 and thawed at the time of transplantation (75% viability). | |
Handle: | http://hdl.handle.net/11577/2454104 | |
Appare nelle tipologie: | 01.01 - Articolo in rivista |
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate
Loading suggested articles...
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.