Purpose: Aims were to verify: (1) whether entire and mw fractioned cell culture media conditioned (CM) by pancreatic cancer (PC) cell lines are able to alter glucose metabolism of isolated and perfused rat hepatocytes (IPRH); (2) the molecular composition of control (NCM) and CM by MALDI analysis; (3) which component is detectable in sera of control subjects (CS, n = 10), patients with chronic pancreatitis (CP, n = 9) or with pancreatic cancer (PC, n = 14) and whether they are associated with diabetes mellitus. Methods: CM were from MIAPaCa2, PSK1, PANC1 and CAPAN1 lines. CM and NCM were fractioned (mw !30,000 D and mw !10,000 D). IPRH (male Wistar rats) were incubated with NCM or CM. Results: Lactate production was inhibited when the IPRH were incubated with the CM from all the PC lines. This inhibitory effect was reproduced also by CM with a mw !10,000 D. MALDI analysis revealed two peptides, of 1,874 D and of 2,030 D, expressed by all CM, and another of 2,726 D expressed by 2/4 PC lines. The 2,030 and the 2,726 D were also found in some patients’ sera. Only the 2,030 D component was found to be associated with the presence of diabetes considering PC patients (Fisher’s exact test: p ! 0.05). Conclusions: The production of a low mw diabetogenic factor is a phenomenon common to several PC lines; a component of 2,030 D seems to be that more significantly correlated with PC associated diabetes.

A 2030 D peptide as the putative pancreatic cancer-associated diabetogenic factor

BASSO, DANIELA;VALERIO, ANNA CANDIDA;GRECO, ELIANA;FOGAR, PAOLA;PEDRAZZOLI, SERGIO;TIENGO, ANTONIO
2001

Abstract

Purpose: Aims were to verify: (1) whether entire and mw fractioned cell culture media conditioned (CM) by pancreatic cancer (PC) cell lines are able to alter glucose metabolism of isolated and perfused rat hepatocytes (IPRH); (2) the molecular composition of control (NCM) and CM by MALDI analysis; (3) which component is detectable in sera of control subjects (CS, n = 10), patients with chronic pancreatitis (CP, n = 9) or with pancreatic cancer (PC, n = 14) and whether they are associated with diabetes mellitus. Methods: CM were from MIAPaCa2, PSK1, PANC1 and CAPAN1 lines. CM and NCM were fractioned (mw !30,000 D and mw !10,000 D). IPRH (male Wistar rats) were incubated with NCM or CM. Results: Lactate production was inhibited when the IPRH were incubated with the CM from all the PC lines. This inhibitory effect was reproduced also by CM with a mw !10,000 D. MALDI analysis revealed two peptides, of 1,874 D and of 2,030 D, expressed by all CM, and another of 2,726 D expressed by 2/4 PC lines. The 2,030 and the 2,726 D were also found in some patients’ sera. Only the 2,030 D component was found to be associated with the presence of diabetes considering PC patients (Fisher’s exact test: p ! 0.05). Conclusions: The production of a low mw diabetogenic factor is a phenomenon common to several PC lines; a component of 2,030 D seems to be that more significantly correlated with PC associated diabetes.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11577/2454792
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