A 600 m3 of soil were removed from an oil contaminated site and a biopile was assessed. Microbial population dynamics of both resident microflora and newly introduced inoculant strains, were constantly monitored and found associated with oil content fluctuations in soil, as evaluated by gaschromatographic and Infra Red analyses. Due to the heterogeneity of the residual fuel content in the biopile after several months, a study of the metabolic activity of soil microbiota was undertaken by coupling Total Direct Counts (TDC) with CTC (5-cyano-2,3-di-4-tolyl-tetrazolium chloride) reduction assays, indicating that such a determination can usefully be applied as an indicator of the efficiency of the system. Soil DNA extraction and analyses were also undertaken.
Metabolic state of microbial populations as an efficiency index of a biopile
BASAGLIA, MARINA;SQUARTINI, ANDREA;CASELLA, SERGIO
2003
Abstract
A 600 m3 of soil were removed from an oil contaminated site and a biopile was assessed. Microbial population dynamics of both resident microflora and newly introduced inoculant strains, were constantly monitored and found associated with oil content fluctuations in soil, as evaluated by gaschromatographic and Infra Red analyses. Due to the heterogeneity of the residual fuel content in the biopile after several months, a study of the metabolic activity of soil microbiota was undertaken by coupling Total Direct Counts (TDC) with CTC (5-cyano-2,3-di-4-tolyl-tetrazolium chloride) reduction assays, indicating that such a determination can usefully be applied as an indicator of the efficiency of the system. Soil DNA extraction and analyses were also undertaken.Pubblicazioni consigliate
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