A pulsed-field gel electrophoretic method based on contour-clamped homogeneous electric field (CHEF) was developed for the analysis of natural isolates of Rhizobium leguminosarum biovar viciae. The procedure involves the use of 'rare-cutting' endonucleases. The separation of genomic DNA fragments with split runs (τ = 5 s for 15 h followed by τ = 12 s for 9 h) allows a clear definition of profiles with bands ranging from 20-300-kb pairs. Two and a half days are sufficient to reproducibly accomplish the procedure from cell lysis to gel picture. The method can be used for different fast-growing rhizobia.
Pulsed-field electrophoresis in contour-clamped homogeneous electric fields (CHEF) for the fingerprinting of Rhizobium spp.
CORICH, VIVIANA;GIACOMINI, ALESSIO;SQUARTINI, ANDREA;
1991
Abstract
A pulsed-field gel electrophoretic method based on contour-clamped homogeneous electric field (CHEF) was developed for the analysis of natural isolates of Rhizobium leguminosarum biovar viciae. The procedure involves the use of 'rare-cutting' endonucleases. The separation of genomic DNA fragments with split runs (τ = 5 s for 15 h followed by τ = 12 s for 9 h) allows a clear definition of profiles with bands ranging from 20-300-kb pairs. Two and a half days are sufficient to reproducibly accomplish the procedure from cell lysis to gel picture. The method can be used for different fast-growing rhizobia.File in questo prodotto:
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