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EnglishTwo endo-polygalacturonase isoenzymes (PG-II and PG-IV), with masses of 34 and 30 kDa respectively, were purified from soybean hypocotyls infected by Sclerotinia sclerotiorum. The pH optimum for both isoenzymes was about 4.6, but PG-IV exhibited a broader range of pH activity. PG-IV showed a much higher affinity for pectin than did PG-II. PG-II hydrolyzed polygalacturonic acid in a more random fashion than PG-IV. Oligouronides produced by PG-II showed a higher phytoalexin elicitor activity. PG-IV produced a large degree of maceration of soybean hypocotyls releasing a significant amount of uronides. The properties of PG-II and PG-IV are discussed in relation to the different ability of the two isoenzymes to elicit glyceollin in soybean.
| Titolo: | CHARACTERIZATION OF 2 SCLEROTINIA-SCLEROTIORUM POLYGALACTURONASES WITH DIFFERENT ABILITIES TO ELICIT GLYCEOLLIN IN SOYBEAN |
| Autori: | |
| Data di pubblicazione: | 1992 |
| Rivista: | |
| Abstract: | Two endo-polygalacturonase isoenzymes (PG-II and PG-IV), with masses of 34 and 30 kDa respectively, were purified from soybean hypocotyls infected by Sclerotinia sclerotiorum. The pH optimum for both isoenzymes was about 4.6, but PG-IV exhibited a broader range of pH activity. PG-IV showed a much higher affinity for pectin than did PG-II. PG-II hydrolyzed polygalacturonic acid in a more random fashion than PG-IV. Oligouronides produced by PG-II showed a higher phytoalexin elicitor activity. PG-IV produced a large degree of maceration of soybean hypocotyls releasing a significant amount of uronides. The properties of PG-II and PG-IV are discussed in relation to the different ability of the two isoenzymes to elicit glyceollin in soybean. |
| Handle: | http://hdl.handle.net/11577/2459577 |
| Appare nelle tipologie: | 01.01 - Articolo in rivista |
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