Polysomes were isolated from aortas of 17-day-old chick embryos, and the syntehsis of the nascent polypeptide chains was completed in vitro. When a mixture of a labeled amino acids found in elastin was used, the major radioactive product obtained was of molecular weight about 70,000 and was similar to elastin by several criteria. The 70,000 molecular weight product was extractable in propanol-butanol, it was not labeled with (35)S-methionine, and it was precipitated by antibodies against elastin. Polypeptides lager than 70,000 molecular weight were also synthesized, but these lager polypeptides incorporated relatively small amounts of (14)C-valine, and they appeared to represent pro-alpha chains of procollagen. These results suggest that the major gene product for elastin has a molecular weight of about 70,000.
Synthesis of an elastin component of molecular weight about 70,000 by polysomes from chick embryo aortas.
BRESSAN, GIORGIO;
1978
Abstract
Polysomes were isolated from aortas of 17-day-old chick embryos, and the syntehsis of the nascent polypeptide chains was completed in vitro. When a mixture of a labeled amino acids found in elastin was used, the major radioactive product obtained was of molecular weight about 70,000 and was similar to elastin by several criteria. The 70,000 molecular weight product was extractable in propanol-butanol, it was not labeled with (35)S-methionine, and it was precipitated by antibodies against elastin. Polypeptides lager than 70,000 molecular weight were also synthesized, but these lager polypeptides incorporated relatively small amounts of (14)C-valine, and they appeared to represent pro-alpha chains of procollagen. These results suggest that the major gene product for elastin has a molecular weight of about 70,000.Pubblicazioni consigliate
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