Abstract of Proceedings- 2011 http://www.fissr.dbabstract.com/US/US_Upd.aspx?IDA=1450 06/04/2011 Gruppo Imatìka 1° Congresso FISSR – Riccione - 26-28 Maggio 2011 EVALUATION OF PREGNANCY RATE IN CYCLES OF FREEZING IVF ZYGOTES USING THE FREEZING OF EMBRYOS R. Laganara1, M. Gangemi1, M. L. Coronella1, A. Riva', G. B. Nardelli1 Dpt Gynecological Science and Human Reoproduction – University of Padova-Italy The optimization results of a cycle of in vitro fertilization is achieved with a high cumulative rate of pregnancy for each pick-up. To achieve this result it is necessary to adopt a program of cryopreservation that maximizes the results. Freezing can be done at the stage of pronuclei (zygote) or to cells (embryos). In some countries, like Switzerland and Germany, freezing at the pronuclear stage is only allowed by law, considering that this is acceptable from an ethical point of view. Much literature also highlights the best pregnancy rate (PR) obtained with the freezing of zygotes compared to that of embryos. We wanted to verify this finding in our series. Were evaluated retrospectively 260 cycles of transference of embryos thawed, divided into two groups of 130: group 1 includes frozen embryos to the stage of pronuclei (PN) (day 1 after the egg retrieval; 20-22 hours after insemination) and group 2 embryos frozen at the stage of 2-4 cells (day 2 after egg retrieval, 44-46 hours after insemination). The transference was always done on natural cycle, with ovulation by plasmatic monitoring of E2, progesterone and LH ; the zygotes were thawed the day before the transfer and maintained in culture for 24 hours already cleaved embryos were thawed the day of transfer. According to the age of the patient and the number of embryos available have been transferred from 1 to 3 embryos. We evaluated the PR by determination of HCG 14 days after the transfer and viewing of an amniotic sac with fetal cardiac activity after 28 days of transfer. The PR was higher in group 1 (19.23% - 25/130) than in group 2 (15.38% -20 / 130), with a difference not statistically significant (P = Q, 3 \). The freezing to the zygote stage provides a PR greater than the freezing stage of embryo cleaved, albeit with a difference not statistically significant. Whereas, in the zygote, the fusion of genetic material of two parents in the genetic makeup of the new embryo has not yet occurred, the cryopreservation of zygotes can be more easily accepted by the ethical point of view, as well as provide better results

Evaluation of pregnancy rate in cycles of freezing IVF zygotes using the freezing of embryos

GANGEMI, MICHELE;NARDELLI, GIOVANNI BATTISTA
2011

Abstract

Abstract of Proceedings- 2011 http://www.fissr.dbabstract.com/US/US_Upd.aspx?IDA=1450 06/04/2011 Gruppo Imatìka 1° Congresso FISSR – Riccione - 26-28 Maggio 2011 EVALUATION OF PREGNANCY RATE IN CYCLES OF FREEZING IVF ZYGOTES USING THE FREEZING OF EMBRYOS R. Laganara1, M. Gangemi1, M. L. Coronella1, A. Riva', G. B. Nardelli1 Dpt Gynecological Science and Human Reoproduction – University of Padova-Italy The optimization results of a cycle of in vitro fertilization is achieved with a high cumulative rate of pregnancy for each pick-up. To achieve this result it is necessary to adopt a program of cryopreservation that maximizes the results. Freezing can be done at the stage of pronuclei (zygote) or to cells (embryos). In some countries, like Switzerland and Germany, freezing at the pronuclear stage is only allowed by law, considering that this is acceptable from an ethical point of view. Much literature also highlights the best pregnancy rate (PR) obtained with the freezing of zygotes compared to that of embryos. We wanted to verify this finding in our series. Were evaluated retrospectively 260 cycles of transference of embryos thawed, divided into two groups of 130: group 1 includes frozen embryos to the stage of pronuclei (PN) (day 1 after the egg retrieval; 20-22 hours after insemination) and group 2 embryos frozen at the stage of 2-4 cells (day 2 after egg retrieval, 44-46 hours after insemination). The transference was always done on natural cycle, with ovulation by plasmatic monitoring of E2, progesterone and LH ; the zygotes were thawed the day before the transfer and maintained in culture for 24 hours already cleaved embryos were thawed the day of transfer. According to the age of the patient and the number of embryos available have been transferred from 1 to 3 embryos. We evaluated the PR by determination of HCG 14 days after the transfer and viewing of an amniotic sac with fetal cardiac activity after 28 days of transfer. The PR was higher in group 1 (19.23% - 25/130) than in group 2 (15.38% -20 / 130), with a difference not statistically significant (P = Q, 3 \). The freezing to the zygote stage provides a PR greater than the freezing stage of embryo cleaved, albeit with a difference not statistically significant. Whereas, in the zygote, the fusion of genetic material of two parents in the genetic makeup of the new embryo has not yet occurred, the cryopreservation of zygotes can be more easily accepted by the ethical point of view, as well as provide better results
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11577/2484647
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact