A direct method able to determine residues of macrolide antibiotics erythromycin, tylosin and tilmicosin in whole eggs is here presented. This method proposed is based on a one-step extraction followed by liquid chromatography-tandem mass spectrometry. Analytes were extracted with acetonitrile from 1 g of egg. No cleanup was found to be necessary. Extraction was effective, since absolute recovery of the analyte in egg at the level of 5 ng/g for tilmicosin and at the Maximum Residue Limit (MRL) for erythromycin and tylosin were 89-103%. Based on the EU Commission Decision 2002/657/EC1, the method was in-house validated in terms of ruggedness, specificity, linearity, within-laboratory reproducibility, decision limit (CCα) and detection capability (CCβ). The within-laboratory reproducibility (n= 18) at MRL level was in the 12-13% range. After validation, a short evaluation of the depletion of erythromycin in eggs was conducted after administration of this drug to laying hens.

Analysis of macrolide antibiotics in eggs validated according to European Commission Decision 2002/657/EC: a rapid extraction followed by liquid chromatography –tandem mass spectrometry

BOGIALLI, SARA;
2009

Abstract

A direct method able to determine residues of macrolide antibiotics erythromycin, tylosin and tilmicosin in whole eggs is here presented. This method proposed is based on a one-step extraction followed by liquid chromatography-tandem mass spectrometry. Analytes were extracted with acetonitrile from 1 g of egg. No cleanup was found to be necessary. Extraction was effective, since absolute recovery of the analyte in egg at the level of 5 ng/g for tilmicosin and at the Maximum Residue Limit (MRL) for erythromycin and tylosin were 89-103%. Based on the EU Commission Decision 2002/657/EC1, the method was in-house validated in terms of ruggedness, specificity, linearity, within-laboratory reproducibility, decision limit (CCα) and detection capability (CCβ). The within-laboratory reproducibility (n= 18) at MRL level was in the 12-13% range. After validation, a short evaluation of the depletion of erythromycin in eggs was conducted after administration of this drug to laying hens.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2485278
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