Further study of the mitochondrial transport of spermine (Toninello et al. (1988) J. Biol. Chem. 263, 19407) shows that, after loading rat liver mitochondria with [14C]sperminc and [32P]phosphate, these components are released together into the surrounding medium by adding mersalyl or N-ethylmaleimide. On later addition of dithioerythritol, both are recaptured, but if acetate or nigericin are added instead, only spermine re-enters and there is continued export of phosphate. This bidirectional transport of spermine in and out mitochondria is driven, respectively, by membrane potential and pH gradient at constant protonmotive force. Results using [14C]spermine or [32P]phosphate, in conjunction with the their unlabelled isomersand with or without carbonyl cyanide/p-trifuloromethoxyphenylhydrazone (FCCP) present suggest that there is a continuous energy-dependent efflux-influx cycling of spermine and phosphate.

Bidirectional transport of spermine in rat liver mitochondria

TONINELLO, ANTONIO;DALLA VIA, LISA
1992

Abstract

Further study of the mitochondrial transport of spermine (Toninello et al. (1988) J. Biol. Chem. 263, 19407) shows that, after loading rat liver mitochondria with [14C]sperminc and [32P]phosphate, these components are released together into the surrounding medium by adding mersalyl or N-ethylmaleimide. On later addition of dithioerythritol, both are recaptured, but if acetate or nigericin are added instead, only spermine re-enters and there is continued export of phosphate. This bidirectional transport of spermine in and out mitochondria is driven, respectively, by membrane potential and pH gradient at constant protonmotive force. Results using [14C]spermine or [32P]phosphate, in conjunction with the their unlabelled isomersand with or without carbonyl cyanide/p-trifuloromethoxyphenylhydrazone (FCCP) present suggest that there is a continuous energy-dependent efflux-influx cycling of spermine and phosphate.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2485377
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