Two groups of 42 rabbits were fed either a diet supplemented or not with Spirulina Duration, from weaned (35d old) to 11 weeks of age. Samples of caecal content were collected at the 14th, 28th and 48th day of the supplementation, to evaluate the effect on the bacterial diversity of microbiota using molecular tools. After bacterial DNA extraction, the quantity of bacteria, belonging to phylum Firmicutes and Bacteroidetes were determined by qPCR reactions. The copy number of the two phyla did not significantly evolved from 49 to 83d of age, and ranged between 87000 and 118000. The amount of Bacteroidetes and Clostridium group (Clostridium leptum and Clostridium coccoides), belonging to the Firmicutes phylum, were not significant modified by Spirulina dietary supplementation.
DIETARY SUPPLEMENTATION OF SPIRULINA (Arthrospira platensis) AND THYME (Thymus vulgaris). PART 3: EFFECT ON BACTERIAL DIVERSITY IN THE CAECUM OF RABBITS.
DALLE ZOTTE, ANTONELLA;
2012
Abstract
Two groups of 42 rabbits were fed either a diet supplemented or not with Spirulina Duration, from weaned (35d old) to 11 weeks of age. Samples of caecal content were collected at the 14th, 28th and 48th day of the supplementation, to evaluate the effect on the bacterial diversity of microbiota using molecular tools. After bacterial DNA extraction, the quantity of bacteria, belonging to phylum Firmicutes and Bacteroidetes were determined by qPCR reactions. The copy number of the two phyla did not significantly evolved from 49 to 83d of age, and ranged between 87000 and 118000. The amount of Bacteroidetes and Clostridium group (Clostridium leptum and Clostridium coccoides), belonging to the Firmicutes phylum, were not significant modified by Spirulina dietary supplementation.
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