Three analogues of the tridecapeptide amide H-Leu-(Glu)5-Ala-Tyr-Gly-Nle-Asp-Phe-NH2 were synthetized with α-deuterated glutamate residues in specific positions in order to assign unambiguously the 1H nmr spectrum of the parent peptide in water and in water-trifluoroethanol mixtures. The synthetic route is described and the assignment illustrated. A previous, tentative assignment based solely on indirect evidence [Mammi, S., Mammi, N. J. & Peggioa, E. (1988) Biochemistry27, 1374–1379] was partially modified.

Synthesis and H-1-nmr Studies of Alpha-deuterated Analogs of Des-trp1, Nle12-human Minigastrin

MAMMI, STEFANO;PEGGION, EVARISTO
1990

Abstract

Three analogues of the tridecapeptide amide H-Leu-(Glu)5-Ala-Tyr-Gly-Nle-Asp-Phe-NH2 were synthetized with α-deuterated glutamate residues in specific positions in order to assign unambiguously the 1H nmr spectrum of the parent peptide in water and in water-trifluoroethanol mixtures. The synthetic route is described and the assignment illustrated. A previous, tentative assignment based solely on indirect evidence [Mammi, S., Mammi, N. J. & Peggioa, E. (1988) Biochemistry27, 1374–1379] was partially modified.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11577/2510200
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