Reversal by phospholipids of the oligomycin induced inhibition of membrane associated adenosintriphosphatases.

The inhibitory effect of oligomycin on sodium, potassium-stimulated ATPase and on mitochondrial ATPase was removed by the addition of purified phospholipids to the incubation medium. Ouabain effect was not modified. Phospholipids with isoelectric ionic portion (particularly lecithins) were found more active than phosphatidylethanolamines and bovine brain phosphatidylserine. Differences in the activity of lecithins from various sources were also seen. This indicates that the composition of hydrophobic fatty acid chains influences the reversal of oligomycin effect. The antagonism by the external added phospholipids may reflect the affinity of oligomycin with the phospholipids of the enzyme preparation. © 1970.