Protein kinase CK2 is a pleiotropic serine/threonine kinase responsible for the generation of a substantial proportion of the human phosphoproteome. CK2 is generally found as a tetramer with two catalytic, alpha and alpha' and two non catalytic beta subunits. CK2 alpha C-terminal tail phosphotylation is regulated during the mitotic events and the absence of these phosphosites in alpha' suggests an isoform specialization. We used a proteomic approach to identify proteins specifically phosphorylated by a CK2 alpha phosphomimetic mutant, CK2 alpha T344ET360ES362ES370E (CK2 alpha 4E), in human neuroblastoma SKNBE cellular extract. One of these proteins is lysine-specific demethylase 1 (LSD1 or KDM1A), an important player of the epigenetic machinery. LSD1 is a FAD-dependent amine oxidase and promotes demethylation of lysine 4 and lysine 9 of mono- and di-methylated histone H3. We found that LSD1 is a new substrate and an interacting partner of protein kinase CK2. Three CK2 phosphosites, (Ser131, Ser137 and Ser166) in the N-terminal region of LSD1 have been identified. This domain is found in all chordates but not in more ancient organisms and it is not essential for LSD1 catalytic event while it could modulate the interaction with CK2 and with other partners in gene repressing and activating complexes. Our data support the view that the phosphorylation of the N-terminal domain by CK2 may represent a mechanism for regulating histone methylation, disclosing a new role for protein kinase CK2 in epigenetics. (C) 2014 Elsevier B.V. All rights reserved.

The lysine-specific demethylase 1 is a novel substrate of protein kinase CK2

COSTA, ROBERTO;ARRIGONI, GIORGIO;COZZA, GIORGIO;LOLLI, GRAZIANO;BATTISTUTTA, ROBERTO;PINNA, LORENZO;SARNO, STEFANIA
2014

Abstract

Protein kinase CK2 is a pleiotropic serine/threonine kinase responsible for the generation of a substantial proportion of the human phosphoproteome. CK2 is generally found as a tetramer with two catalytic, alpha and alpha' and two non catalytic beta subunits. CK2 alpha C-terminal tail phosphotylation is regulated during the mitotic events and the absence of these phosphosites in alpha' suggests an isoform specialization. We used a proteomic approach to identify proteins specifically phosphorylated by a CK2 alpha phosphomimetic mutant, CK2 alpha T344ET360ES362ES370E (CK2 alpha 4E), in human neuroblastoma SKNBE cellular extract. One of these proteins is lysine-specific demethylase 1 (LSD1 or KDM1A), an important player of the epigenetic machinery. LSD1 is a FAD-dependent amine oxidase and promotes demethylation of lysine 4 and lysine 9 of mono- and di-methylated histone H3. We found that LSD1 is a new substrate and an interacting partner of protein kinase CK2. Three CK2 phosphosites, (Ser131, Ser137 and Ser166) in the N-terminal region of LSD1 have been identified. This domain is found in all chordates but not in more ancient organisms and it is not essential for LSD1 catalytic event while it could modulate the interaction with CK2 and with other partners in gene repressing and activating complexes. Our data support the view that the phosphorylation of the N-terminal domain by CK2 may represent a mechanism for regulating histone methylation, disclosing a new role for protein kinase CK2 in epigenetics. (C) 2014 Elsevier B.V. All rights reserved.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11577/2828369
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