Following the Biocatalytic Activities of Glucose Oxidase by Electrochemically Cross-Linked Enzyme-Pt Nanoparticles Composite Electrodes

An integrated platinum nanoparticles (NPs)/glucose oxidase (GOx) composite film associated with a Au electrode is used to follow the biocatalytic activities of the enzyme. The film is assembled on a Au electrode by the electropolymerization of thioaniline-functionalized Pt NPs and thioaniline-modified GOx. The resulting enzyme/Pt NPs-functionalized electrode stimulates the O-2 oxidation of glucose to gluconic acid and H2O2. The modified electrode is then implemented to follow the activity of the enzyme by the electrochemical monitoring of the generated H2O2. The effect of the composition of the Pt NPs/GOx crosslinked nanostructures and the optimal conditions for the preparation of the electrodes are discussed.