3D high-resolution hydrogel structures for biological studies

Hydrogels are widely used as matrices for cell growth due to the similarity of their mechanical and diffusivity properties to the extracellular matrix. The microfabrication of hydrogels into arbitrarily complex 3D structures is becoming essential for numerous biological applications. Among the possible additive manufacturing techniques, two-photon lithography stands out for its unique capacity of a direct 3D writing. We report fabrication of 3D high resolution hydrogel gelatin structures by Nanoscribe GT Photonic Professional. According to the degree of polymerization, variable swelling and structure deformation is observed. As a preliminary investigation to any biological application, the developed 3D hydrogels are tested against the requirements of cytocompatibility, promotion of cell adhesion and diffusion using BJ cell lines.