Estimation of fetal lung development using quantitative analysis of ultrasonographic images in normal canine pregnancy

We investigated the quantitative analysis of sonographic images to predict fetal lung maturity of the canine foetus in normal pregnancy. Twelve bitches were recruited in the present study. Serial ultraso- nographic exams were performed at three pre-determined time periods corresponding to the pseudo- glandular (40-48 days of pregnancy), canalicular (49-56 days of pregnancy) and saccular phase (57-63 days of pregnancy) of lung development. Mean grey level (MGL) and the standard deviation of the histogram (SDH) of fetal lung and liver sonographic images were measured with dedicated software. The lung-to-liver ratio (LLR) for both parameters was also calculated. Measurements were taken on the two caudal-most foetuses and then averaged. SDH did not show any statistically significant difference bEtween the three time periods in the lungs or in the liver. MGL measured in the lungs significantly increased in the first period and reached a plateau during the last two periods. Liver echogenicity wasconstant during the first two periods and significantly increased during the last week of gestation. TheLLR of MGL significantly decreased during the last week of pregnancy. The LLR was a very good test to detect fetal lung maturity (area under the receiver operator curve (AUROC) =0.875); using a cut-off value of LLR<1.541, sensitivity was 83.33% and specificity was 83.33%, positive likelihood ratio=5. LLR of MGL is an accurate test to estimate lung development in normal canine pregnancies.